The objective of this study was to compare the effects of maturation on physical fitness adaptations to a twice weekly, 7-week plyometric drop jump training program. Seventy-six young male soccer players (aged 10-16 years) participated in this randomized controlled trial. Before and after the intervention, a physical fitness test battery was applied (countermovement jump; drop jump from 20 to 40 cm; 5 multiple bounds test; 20-m sprint time; change of direction speed; 2.4-km running time-trial; 5 repetition maximum [RM] squat; and maximal kicking distance). Participants were randomly divided into an active soccer-control group (CG) with Tanner stage maturation of 1-3 (CG-early; n 5 16) or Tanner stage 4-5 (CG-late; n 5 22), and to plyometric drop jump training groups with Tanner stage 1-3 (plyometric jump training [PJT]-early; n 5 16) or 4-5 (PJT-late; n 5 22). The analysis of variance and effect size (ES) measures revealed that when compared with their age-matched controls, the PJT-early (ES 5 0.39-1.58) and PJT-late (ES 5 0.21-0.65) groups showed greater improvements (p , 0.05) in sprint time, 2.4-km running time-trial, change of direction speed, 5RM squat, jumping, and kicking distance. The PJT-early exceeded the PJT-late group with greater (p , 0.05) improvements in drop jump from 20 cm (ES 5 1.58 vs. 0.51) and 40 cm (ES 5 0.71 vs. 0.4) and kicking distance (ES 5 0.95 vs. 0.65). Therefore, a 7-week plyometric drop jump training program was effective in improving physical fitness traits in both younger and older male youth soccer players, with greater jumping and kicking adaptations in the less-mature athletes.